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Test No. 442E: In Vitro Skin Sensitisation [Elektronische Ressource]: Human Cell Line Activation Test (h-CLAT)

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Titel: Test No. 442E: In Vitro Skin Sensitisation [Elektronische Ressource]: Human Cell Line Activation Test (h-CLAT) / Organisation for Economic Co-operation and Development
Körperschaft: Organisation for Economic Co-operation and Development
Link: Volltext
Ort / Verlag, Jahr: Paris: OECD Publishing, 2016
Umfang: 1 online resource (21 pages).
Verknüpfte Werke: OECD Guidelines for the Testing of Chemicals, Section 4
ISBN: 9789264264359; 9264264353
Typ: Online vorhanden Typ Online vorhanden
Sonstiges:
  • Online-Ressource [Kann nicht per Fernleihe bestellt werden!]

Zitierlink zu diesem Treffer: http://www.digibib.net/permalink/836/FHBM-x/DE-836:ocn958185271

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Abstract:
  • The present Test Guideline addresses the human health hazard endpoint skin sensitisation, following exposure to a test chemical. Skin sensitisation refers to an allergic response following skin contact with the tested chemical, as defined by the United Nations Globally Harmonized System of Classification and Labelling of Chemicals (UN GHS). This Test Guideline (TG) provides an in vitro procedure (the human cell Line Activation Test h-CLAT method) used for supporting the discrimination between skin sensitisers and non-sensitisers in accordance with the UN GHS. The h-CLAT method is proposed to address the third key event of the skin sensitisation AOP by quantifying changes in the expression of cell surface markers associated with the process of activation of monocytes and dendritic cells (DC) (i.e. CD86 and CD54), in the human monocytic leukaemia cell line THP-1, following exposure to sensitising test chemical. These surface molecules are typical markers of monocytic THP-1 activation and may mimic DC activation, which plays a critical role in T-cell priming. The changes of surface marker expression are measured by flow cytometry following cell staining with fluorochrome-tagged antibodies. The relative fluorescence intensity of surface markers compared to solvent/vehicle control are calculated and used in the prediction model, to support the discrimination between sensitisers and non-sensitisers.
DOI: http://dx.doi.org/10.1787/9789264264359-en